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RenaLife 培養基RenaLife Medium Complete Kit

更新日期:2014年03月21日 瀏覽次數:
名稱:RenaLife 培養基RenaLife Medium Complete Kit

貨號:LL-0025

英文名稱:RenaLife Medium Complete Kit

價格:1824.00RMB

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RenaLife? medium is optimized for the low-serum culture of Human Renal Epithelial Cells. RenaLife? supports the growth of these cells over a period of at least 15 population doublings.

RenaLife? Basal Medium contains no antimicrobials and no phenol red. These supplements are available from Lifeline? but are not required for eukaryotic cell proliferation.

  • Kit format for formulation flexibility:  RenaLife? culture medium is offered in a kit format composed of RenaLife? Basal Medium (500mL) and associated supplements and growth factors called “LifeFactors?.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective packaging:  RenaLife? Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors?(growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

Human Renal Epithelial Cell Culture Medium

ISO-Style Testing for Guaranteed Consistency and Reproducible Results

Every production lot of RenaLife? Medium is extensively tested using Human Renal Epithelial Cells to ensure lot-to-lot consistency.

  • Sterility Testing:  Negative for bacterial and fungal growth
  • pH: 7.5 +/- 0.2
  • Cell Testing: Rate of proliferation and morphology
  • Osmolality: 315 +/- 10 mOsm
  • Endotoxin: < 0.5 EU/mL

RenaLifeRenal Cortical Epithelial Cell CultureTM Medium grows Human Renal Epithelial Cells through at least 15 population doublings at rates that are comparable to other commercially available low-serum media, while maintaining more characteristic epithelial morphology, e.g., more compact cuboidal shape, high refractive index and clarity of the cytoplasm.

Shown left: Renal Cortical Epithelial Cells, third subculture (100X).

Shown below: gamma-Glutamyltransferase (GGT) assay. The substrate (gamma-glutamyl-p-nitroanilide) and acceptor molecule (glycylglycine) are incubated with the cells in a sodium phosphate buffer for 30 minutes at 37°C. The released product is p-nitroaniline (PNA). The absorbance at 405 nm is measured and compared against a standard curve of PNA. Protein is quantified using a modified Bradford method.

RenaLife renal epithelial medium comparison

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